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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which ends up in chromosome instability, and therefore may lead to tumorigenesis. This protein was found to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a critical position in advertising cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually described. Two more splice variants ended up claimed but detailed nucleotide sequence info is just not nevertheless readily available.
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Blocking peptides are peptides that bind precisely for the concentrate on antibody and block antibody binding. These peptide typically has the epitope identified from the antibody.
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This information outlines additional attainable optimization of mobile and immunoassay parameters to be sure the best possible success are...
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This guideline outlines further possible optimization of mobile and immunoassay parameters to be sure the very best outcomes are...
Antibodies certain to the blocking peptide no longer bind towards the epitope within the concentrate on protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, you can see which staining is particular.
Goal Details Cyclin E belongs to the very conserved cyclin loved ones, whose users are characterised by a spectacular periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add for the temporal coordination of every mitotic party. Cyclin E kinds a posh with and features as being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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